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Image Search Results
Journal: bioRxiv
Article Title: Longitudinal analysis of retinal cell state transitions in RB1 -deficient retinal organoids reveals the nascent cone precursors are the earliest cell-origin of human retinoblastoma
doi: 10.1101/2025.03.13.642537
Figure Lengend Snippet: (A) Schematic outline of the generation of 1st and 2nd xenograft. (B) Representative optical coherence tomography (OCT) images of engrafted eyes at 10 WAI from RB1 −/− ROs. An obvious highly reflective tumor was observed. (C) Representative slit-lamp images of engrafted eyes at 13 WAI from RB1 −/− ROs. Whitish tumor cells were observed in anterior chambers. (D) Representative microscopic image of tumor mass derived from 1st xenograft. (E) Representative H&E staining images of 1st xenograft from RB1 −/− ROs. The dash lines indicate the rosette structure. (F) Representative H&E staining images of 2nd xenograft with injection of 1st tumor cells. No rosettes were observed. (G) Representative immunostaining for Ki67 and quantification of Ki67 + cells in 1st and 2nd xenograft. ** P < 0.01 vs. 1st xenograft, n = 3. (H) Representative immunostaining for human specific marker (STEM121), cone markers (RCVRN, RXRγ) and Rb markers (SYK, p16 INK4a ) in 1st and 2nd tumor. (I) Representative immunostaining for L/M opsin in 1st and 2nd tumor. Scale bars = 200 μm (D), 500 (left), 50 (right) μm (E, F) and 50 μm (G, H, I).
Article Snippet: Spectral domain optical coherence tomography (SD-OCT) with an
Techniques: Tomography, Derivative Assay, Staining, Injection, Immunostaining, Marker